Composite

Part:BBa_K2686006

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-10-08)
Revision as of 13:26, 8 October 2018 by Registry (Talk | contribs)

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Encapsulin protein with HexaHistidine insert, OT1 peptide at C terminus and a sfGFP-tag protein.

This Composite part consists of an encapsulin protein with a HexaHistidine linker and an OVA peptide fused to the C terminus, followed by a T7 RBS and a sfGFP protein with a 15 amino acid long peptide tag. This peptide tag allows the sfGFP to bind to the encapsulin's interior surface

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 902
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 492
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 902
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 902
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426
    Illegal SapI.rc site found at 457
    Illegal SapI.rc site found at 955


[edit]
Categories
Parameters
None