Coding

Part:BBa_K2653000

Designed by: Jiaxin Ma   Group: iGEM18_NUDT_CHINA   (2018-10-07)
Revision as of 01:47, 8 October 2018 by Helen M (Talk | contribs)


trim21

Trim21 is a type of E3 ubiquitin ligase that has been discovered in many eukaryotic cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 873
    Illegal BamHI site found at 1411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 161
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1251


Design Notes

There is 2 domains for PstI with the sequence of our Trim21, so we carried out point mutation to avoid this.


Source

Its sequence come from articles.

References

[edit]
Categories
Parameters
None