Part:BBa_K2665011

TDH3-Mangrin-6xHis-CYC

This part coded salt tolerance protein, Mangrin form mangrove. This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts. Mangrin is a shaperon like protein and enhances salt tolerance of Escherichia coli, Yeast, and Tobacco Cells. We used this part for improving halotorelance of yeast.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 842
  Illegal BamHI site found at 936
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

In order to demonstrate the function of the Rev protein and RRE, we fused an RRE [1] to U6 snRNA, which has a complex structure and is not transported by the TAP / p15 pathway. In Xenopus oocytes, we ascertained whether this RNA was exported from the nucleus in a Rev-dependent manner.

Reference

[1] A. Yamada, T. Saitoh, T. Mimura et al. (2002) Expression of Mangrove Allene Oxide Cyclase Enhances Salt Tolerance in Escherichia coli, Yeast, and Tobacco Cells, Plant and cell physiology 903-910