Coding
LaG16

Part:BBa_K2549003:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-01)
Revision as of 03:24, 7 October 2018 by Wave (Talk | contribs)


anti-GFP (LaG16)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Two copies of LaG16 were fused to form LaG2 (Part:Part:BBa_K2549002) using a flexible glycine-rich peptide linker[1].

Source

from IDT(gBlock), codon optimized for human

References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170