Part:BBa_K2555002
pBAD-RBS-CueR
This construct contains pBAD (L-arabinose inducing promoter) with RBS and CueR (a transcription factor that binds onto pCopA). E. coli cells use CueR to regulate the cytoplasmic copper concentration. Under different copper concentrations, CueR behaves as a net activator or a net repressor. Arabinose concentation controls the expression of Cue R in the construct. This part is used for construction of BBa_K2555004.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1311
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1250
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1085
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1067
References
(1)Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472.
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