Part:BBa_J70004

BioBasic Cloning Plasmid pUC57

Cloning plasmid used by BioBasic for iGEM parts. This plasmid does not have a standard BioBrick cloning site. It is Ampicillin resistant.

Additional Information (from BioBasic):

• CAP protein binding site - 615-578 (compl. strand);
• mRNA (LacZ) starts at nt position 531 (compl. strand);
• lac repressor binding site -531-511 (compl. strand).

Plasmid pUC57, 2710 bp in length, is a derivative of pUC19. pUC57 MCS contains 6 restriction sites with protruding 3'-ends, that are resistant to E.coli exonuclease III. This vector is designed for cloning and generation of ExoIII deletions. The exact position of genetic elements is shown on the map (termination codons included). DNA replication initiates at position 890 (+/- 1) and proceeds in indicated direction. The bla gene nucleotides 2510-2442 (compl. strand) code for a single peptide.

The map (below) shows enzymes that cut pUC57 DNA once. Enzymes produced by Fermentas are shown in blue. The coordinates refer to the position of the first nucleotide in each recognition sequence.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 396
  Illegal XbaI site found at 425
  Illegal PstI site found at 453
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 396
  Illegal PstI site found at 453
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 396
  Illegal BamHI site found at 435
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 396
  Illegal XbaI site found at 425
  Illegal PstI site found at 453
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 396
  Illegal XbaI site found at 425
  Illegal PstI site found at 453
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1790
  Illegal SapI site found at 707