Part:BBa_J61009

pAC-LuxGFP

pAC-LuxGFP places GFP under the wildtype Vibrio lux device. It's behavior is described in the cytometry data below:

This data, and more on this construct are described in:
[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WK7-4HJRXGD-4&_user=4420&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000059607&_version=1&_urlVersion=0&_userid=4420&md5=e4d8a151375af5f5d30743b82ba81019 Environmentally Controlled Invasion of Cancer Cells by Engineered Bacteria]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1733
  Illegal EcoRI site found at 2688
  Illegal XbaI site found at 588
  Illegal XbaI site found at 5084
  Illegal PstI site found at 138
  Illegal PstI site found at 2498
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1733
  Illegal EcoRI site found at 2688
  Illegal NheI site found at 2657
  Illegal NheI site found at 4242
  Illegal PstI site found at 138
  Illegal PstI site found at 2498
  Illegal NotI site found at 6
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1733
  Illegal EcoRI site found at 2688
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1733
  Illegal EcoRI site found at 2688
  Illegal XbaI site found at 588
  Illegal XbaI site found at 5084
  Illegal PstI site found at 138
  Illegal PstI site found at 2498
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1733
  Illegal EcoRI site found at 2688
  Illegal XbaI site found at 588
  Illegal XbaI site found at 5084
  Illegal PstI site found at 138
  Illegal PstI site found at 2498
  Illegal AgeI site found at 4328
  Illegal AgeI site found at 4652
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2781
  Illegal BsaI site found at 2959
  Illegal BsaI.rc site found at 2423