Composite

Part:BBa_K2669000:Experience

Designed by: Joseph Caponi   Group: iGEM18_Uppsala   (2018-09-25)
Revision as of 11:38, 30 September 2018 by Jjc0000 (Talk | contribs)


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Applications of BBa_K2669000

After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. Please note: The exact procedure can be found <a href="http://2018.igem.org/wiki/index.php?title=Team:Uppsala/UnaG">here</a> Bilirubin tests (addition of a small amount of bilirubin to samples) allowed us to see if the UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin. </p>

"T--Uppsala--UnaG_Comparison.png"

Figure 2: Bilirubin test before/after affinity chromatography. Going from right to left the samples are:

  • Lysed sample of the “bad” part before AC
  • Lysed sample of the “good” part before AC
  • "Bad" part after AC
  • "Good" part after AC

User Reviews

UNIQ44a1eb85ede6e40e-partinfo-00000000-QINU UNIQ44a1eb85ede6e40e-partinfo-00000001-QINU