Coding

Part:BBa_K2686001

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-09-30)
Revision as of 11:38, 30 September 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Encapsulin protein

This is a BioBrick containing the sequence for Thermotoga maritima encapsulin, a bacterial protein nanocompartment which self assembles to form a 60-mer. The part can be used to deliver cargo, both on the outer surface of the nanoparticle by fusing a peptide in between the 139/140 Amino Acids as well as the protein's C terminus, or alternately by attaching drugs using 1,4 Michael additions (Thiol -Maleimide reactions) to a cysteine present on the surface of each subunit. Cargo proteins can also be loaded inside the nanocage using a tag binding to Encapsulin's interior surface.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 375
    Illegal SapI.rc site found at 406


[edit]
Categories
Parameters
None