Part:BBa_K2669001

Bilirubin-inducible UnaG+6xHis-tag+Flexible linker

WORK IN PROGRESS

This part is a correction of the Uppsala 2016’s Part:BBa_K2003011, which we noticed had a misplaced start codon. This misplaced start codon caused two major problems:

1) This would lead to a reduction in UnaG expression due to a large space being created between the RBS and the start codon

2) This would lead to no histidine tag being expressed

We have shown that our new improved part does in fact express a histidine tag and has higher expression levels than the old part Part:BBa_K2003011.

Background

UnaG is a unique chromoprotein since it expresses fluorescent signal when in contact with bilirubin. This means (unlike most other chromoproteins) it can be used as a reporter in anaerobic environments or potentially in environments where bilirubin is naturally present, such as the intestines.

Source

This part contains the sequence from Part:BBa_K2003011, with a change to the start codon location. The original source of UnaG is from the paper “A Bilirubin-Inducible Fluorescent Protein from Eel Muscle” by Kumagai A et. al, which characterized the UnaG protein from the muscle of a species of Japanese eel.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]