Composite
Part:BBa_K2598029
Designed by: Matthew Gao Group: iGEM18_UCAS-China (2018-09-25)
bFMO under T7 RNAP sigma fragment T3-regulated promoter pT3
This part contains bFMO,which can catalyse tryptophan and render it blue.The promoter pT3 can be induced by T7 RNAP sigma fragment by directing core fragment to the promoter. A strong terminator is added to avoid recombination
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 186
Illegal AgeI site found at 209
Illegal AgeI site found at 513
Illegal AgeI site found at 843 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |