Coding

Part:BBa_K2598011

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-09-22)
Revision as of 13:11, 22 September 2018 by Registry (Talk | contribs)

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T7 RNAP sigma fragment CGG

This is a T7 RNAP sigma pragment obtained by Segallshapiro T H et al. It can combine to non-active T7 RNAP core fragment (BBa_K2598001) and direct it to specific promoter pCGG(BBa_K2598023)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 578
    Illegal NgoMIV site found at 1193
    Illegal NgoMIV site found at 1202
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None