Coding

Part:BBa_K2586003:Design

Designed by: Robert Warneke, Janek Meiner   Group: iGEM18_Goettingen   (2018-09-10)
Revision as of 11:11, 13 September 2018 by Dschannek96 (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Design Notes

In order to avoid the PstI restriction site, the Adenin at position 1135 was changed to a Thymin.

Source

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.

References

(Malone et. al. 2015): EPSPS gene amplification in glyphosate‐resistant Bromus diandrus

Subtiwiki: [http://subtiwiki.uni-goettingen.de/v3/gene/view/CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1 (aroE)]