Coding
Part:BBa_K2586003:Design
Designed by: Robert Warneke, Janek Meiner Group: iGEM18_Goettingen (2018-09-10)
Revision as of 11:09, 13 September 2018 by Dschannek96 (Talk | contribs)
Design Notes
In order to avoid the PstI restriction site, the Adenin at position 1135 was changed to a Thymin.
Source
This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.
References
(Malone et. al. 2015): EPSPS gene amplification in glyphosate‐resistant Bromus diandrus