RBS
Part:BBa_K2586009:Design
Designed by: Janek Meißner Group: iGEM18_Goettingen (2018-09-13)
RBS-gltT: Combining gltT with a RBS.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 125
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 125
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 125
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 125
Illegal NgoMIV site found at 761
Illegal NgoMIV site found at 1142 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Yet to come
Source
This part was made using BBa_K2586001 and BBa_K2586007 as templates to carry out a PCR. The PCR product was used as insert to clone this part into the required pSB1C3 backbone.