Regulatory

Part:BBa_K2586000:Design

Designed by: Janek Meiner   Group: iGEM18_Goettingen   (2018-09-11)
Revision as of 11:12, 11 September 2018 by FMCommmichau (Talk | contribs) (→‎References)


Palf4: Constitutively active promoter for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design advantage of the promoter is its length of only 30 bp. This makes it easy to introduce into a genetic system. Another advantage lays in the constitutive expression of the promoter: This allows for many sorts of different experiments.


Source

This part was originally described by Gundlach et. al. (Gundlach et. al. 2017). It was artificially designed to carry out experiments with the soil bacterium and model organism Bacillus subtilis.

References

<a href="https://www.ncbi.nlm.nih.gov/pubmed/28420751">Gundlach et. al. 2017</a>


Gundlach et. al. 2017: Control of potassium homeostasis is an essential function of the second messenger cyclic di-AMP in Bacillus subtilis