Coding

Part:BBa_K2586001

Designed by: Robert Warneke, Janek Meißner   Group: iGEM18_Goettingen   (2018-09-10)
Revision as of 06:54, 11 September 2018 by FMCommmichau (Talk | contribs)


GltT: glutamate and glyphosate transporter in B. subtilis

This part is coding for a glutamate and glyphosate transporter. The DNA sequence of the gltT gene is coding for the GltT transporter, which is responsible for glutamate uptake into the cell. We found that GltT is also involved in glyphosate uptake in the Gram-positive model bacterium Bacillus subtilis.

Bacteria lacking the gltT gene are highly resistant to glyphosate because the herbicide is not transported into the cell. This part could be useful to engineer bacteria for the uptake and degradation of the weedkiller.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1123
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part/nominee
//chassis/prokaryote/bsubtilis
Parameters
resistance