Coding

Part:BBa_I757006

Designed by: Freiburg iGEM 2007 team   Group: iGEM07_Freiburg   (2007-10-24)
Revision as of 15:45, 1 November 2007 by Kristian (Talk | contribs)

DHFR2A_109-187_FusionPart

  • seond fragment of the murine split enzyme dihydrofolatereductase (DHFR)
  • purpose: split DHFR activity can be recovered, when fused to interacting domains (Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12141-6; Nat Biotechnol. 1999 Jul;17(7):683-90 ; J Mol Biol. 2000 Jan 21;295(3):627-39)
  • works with first half of split DHFR (part BBa_I757005)
  • NgoMIV / AgeI protein fusion part
  • iGEM Team Freiburg 2007
  • synthetic DNA by GeneArt
  • Part in pGA4 vector (AmpR, ColEI ori)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None