Coding

Part:BBa_K2624002

Designed by: Shuyao Zhou, Junqing Zhang   Group: iGEM18_CPU_CHINA   (2018-08-22)
Revision as of 02:38, 23 August 2018 by Registry (Talk | contribs)

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Nuclear localized NS5B

Nonstructural protein 5B (NS5B) is a viral protein found in the hepatitis C virus (HCV).It is a RNA-dependent RNA polymerase and initiates de novo RNA synthesis with the help of its specific RNA promoters. If you need to generate double stranded RNAs, or substitutional hybridization in situ, this is probably what you are looking for.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 492
    Illegal BglII site found at 1340
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 537


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Parameters
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