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Part:BBa_K2560002

Designed by: Daniel Stukenberg   Group: iGEM18_Marburg   (2018-08-13)
Revision as of 13:04, 13 August 2018 by DanielStukenberg (Talk | contribs)


Phytobrick Entry Vector with GFP dropout

This plasmid can be used to create Phytobricks using a Golden Gate Reaction. A sfGFP dropout helps to distinguish between wrong colonies (green) and correct colonies (white)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1035
    Illegal SapI.rc site found at 193


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Parameters
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