Composite
Part:BBa_K2457005:Design
Designed by: Maria Astolfi, Giovanna Maklouf, Juliana Vitoriano Group: iGEM17_Amazonas_Brazil (2017-09-23)
Standardized pBAD_Cas9 device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1207
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1042
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1024
Design Notes
Cas9</p> The sequence was ordered as two gBlocks from Integrated DNA Technologies and it was assemble by PvuII restriction enzyme site. • NheI (GCTAGC), which in the alanine GCT codon (18% de codon usage) was replaced by GCG (32%) • EcoRI (C-GAATTC), which in arginine CGA codon (30%) was replaced by CGT (24.7%) • First HindIII (AAGCTT) changed to AAACTT • Second HindIII (AAGCTT) changed to AGGCTT • NdeI: CATATG changed to CACATG; CATATG changed to CATACG • NcoI: CCATGG changed to CCGTGG • BamHI: GGATCC changed to GGACCC
Source
descrever
===References===