Translational_Unit

Part:BBa_I737020

Designed by: Vladislav Lavrovsky   Group: iGEM07_Calgary   (2007-10-26)
Revision as of 17:26, 26 October 2007 by Vlad (Talk | contribs)

PoPS->Agarase (Digests agar)

This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. We then cloned it into pSB1A2 using XbaI and SpeI after amplifying with primers that add the BioBrick prefix and suffix. Currently the gene has a EcorRI and a PstI site which limits how it can be assembled.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1861
    Illegal NheI site found at 106
    Illegal NheI site found at 901
    Illegal PstI site found at 284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1861
    Illegal XhoI site found at 1964
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
    Illegal AgeI site found at 368
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1319
    Illegal SapI.rc site found at 1717
    Illegal SapI.rc site found at 1795


[edit]
Categories
//function/biosynthesis
Parameters
None