Device

Part:BBa_K302035

Designed by: Philip Hall   Group: iGEM10_Newcastle   (2010-10-25)
Revision as of 15:00, 1 November 2017 by James zhang (Talk | contribs)

Sucrose-limitation induced kill switch

Encodes a stable non-specific ribonuclease toxin (mazF) and its inhibitory antitoxin (mazE) in Bacillus subtilis. Contains Sucrose sensitive inducer that will only allow coding sequence translation in the presence of sucrose. These genes are used in Bacillus subtilis to provide a toxin-antitoxin kill switch in various stressful conditions. When translation of both genes is turned off by sucrose limitation mazE will be degraded faster than mazF. There is then no inhibiton of mazF, killing the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 363
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


User Reviews

james_zhang

In 2017 SZU-China iGEM team has characterized the output of this part in B.subtilis WB800. The result was documented in the experience page and the main page of BBa_K2232025

[edit]
Categories
//biosafety/kill_switch
Parameters
None