Part:BBa_E0040

green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

GFP (mut3b) [note that this part does not have a barcode]

Usage and Biology

Untagged version of gfp from Repressilator reporter. See the design page for more source information.

Fluorescence wavelengths

Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -

• Excitation max - 501nm
• Emission max - 511nm

Latency

Cormack et al.Cormack report detectable fluorescence within 8 mins. Please add maturation time data for E0040 here.

References

<biblio>

1. Cormack pmid=10659856

</biblio>

Purificaton and Calibration

GFPmut3b can be purified for calibration after the addition of a his-tag. The detailed [http://parts.mit.edu/igem07/index.php/Imperial/Wet_Lab/Protocols/Prot1.6 protocols]and [http://parts.mit.edu/igem07/index.php/Imperial/Wet_Lab/Results/Res1.6 results]for the purification can be found.
The fluorescence of purified GFPmut3B was calibrated in the cell-free chassis. The derived [http://parts.mit.edu/igem07/index.php/Imperial/Wet_Lab/Results/Res1.3 calilbration curve]allows the determination of the concentration of GFPmut3b in the cell-free chassis