Part:BBa_I732103

LacI

Figure 1 Solution structure of a dimer of Lac repressor DNA-binding domain complexed to its natural operator O1 ([http://www.rcsb.org/pdb/explore.do?structureId=1L1M From RCSB])

The sequence produce LaLa repressor protein which is based on natural lacR with only several point mutations. Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly.

Usage and Biology

LaLa could strongly inhibit its corresponding operator which is based on natural lac operator with only several point mutations while it has also weak inhibition to natural lac operator.

(Reference: 1.Lewis, M. (2005), 'The lac repressor.', C R Biol 328 (6), 521--548. 2.Lehming, N.; Sartorius, J.; Oehler, S.; von Wilcken-Bergmann, B. & Müller-Hill, B. Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences. PNAS, 1988, 85, 7947-7951 3. Sartorius, J.; Lehming, N.; Kisters, B.; von Wilcken-Bergmann, B. & Müller-Hill, B. (1989), 'lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchanges.', EMBO J 8(4), 1265--1270.)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]