Cell-free chassis/Vesicle

Revision as of 00:20, 26 October 2007 by Cheuk Ka Tong (Talk | contribs) (→‎Homemade ''E. coli'' S30)

Vesicle-Encapsulation

Cell-Free
Systems (back to Intro) 		Introduction
Chassis
description
Vesicle Specifications.JPG
Preparation
protocol
Preliminary
Testing

Description

Picture of a vesicle expressing GFP. Encapsulated within the vesicle is the Commercial E. coli S30 extract together with the DNA constuct BBa_T9002. The vesicle was seen under a phase-contrast microscope with white light (left picture) and with a filter (right picture).

Method of vesicle-encapsulation

Pictorial representation of method for vesicle-encapsulation based on Engineering Asymmetric Vesicles by Sophie Pautot et al (2003) and Toward an artificial cell based on gene expression in vesicles by Vincent Noireaux et al. (2005).

Please visit our [http://openwetware.org/wiki/IGEM:IMPERIAL/2007/CFS/Design/Protocols OpenWetWare]for the detailed protocol for forming vesicles.

References

<biblio>

  1. 1 pmid=12963816
  2. 2 pmid=16224117

</biblio>