Cell-free chassis/Vesicle

Revision as of 00:13, 26 October 2007 by Cheuk Ka Tong (Talk | contribs) (→‎Vesicle-Encapsulation)

Vesicle-Encapsulation

Homemade E. coli S30

Cell-Free
Systems (back to Intro) 		Introduction
Chassis
description
Preparation protocol
Preliminary Testing

Description

Picture of a vesicle expressing GFP. Encapsulated within the vesicle is the Commercial E. coli S30 extract together with the DNA constuct BBa_T9002. The vesicle was seen under a phase-contrast microscope with white light (left picture) and with a filter (right picture).

Specifications of vesicle-encapsulation

Properties
Definitions
Encapsulation efficiency Measure of the amount of aqeuous contents being encapsulated as a percentage of the total amount of aqueous contents added to make the lipid-oil emulsion.
Vesicle stability Measure of time from vesicle-formation to the point when vesicle pops.
Membrane permeability Measure of the size of the largest particle that can pass through the non-specific protein pore inserted into the phospholipid bilayer.


Method of vesicle-encapsulation

Pictorial representation of method for vesicle-encapsulation based on Engineering Asymmetric Vesicles by Sophie Pautot et al (2003) and Toward an artificial cell based on gene expression in vesicles by Vincent Noireaux et al. (2005).

Please visit our [http://openwetware.org/wiki/IGEM:IMPERIAL/2007/CFS/Design/Protocols OpenWetWare]for the detailed protocol for forming vesicles.

References

<biblio>

  1. 1 pmid=12963816
  2. 2 pmid=16224117

</biblio>