Coding

Part:BBa_K2365034

Designed by: yuanqi jia   Group: iGEM17_NAU-CHINA   (2017-10-14)
Revision as of 13:04, 31 October 2017 by 1314 (Talk | contribs)


ZHD101--Zearalenone degrading enzyme

In our developed assay, the gene encoding ZHD101（a ZEN-degrading enzyme）was cloned. ZHD101, a lactonohydrolase produced by the fungal species Clonostachys rosea, converts ZEN into 1-(3,5-dihydroxy-phenyl)-10-hydroxy-1-undecen- 6-one, which is a markedly less toxic product. In previous studies, ZHD101 has been shown to be successfully expressed by Escherichia coli, Saccharomyces cerevisiae, and rice plants, and the recombinant ZHD101 produced by these genetically modifed organisms efectively degraded ZEN .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 772


ZHD101 DNA.jpg

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Parameters
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