Part:BBa_K354001

PlcR-PapR - Gram+ve Quorum Peptide

The PlcR-PapR fusion peptide has been derived from the Bacillus cereus quorum signalling system. This group of Gram-positive bacteria employs the PlcR quorum system to initiate virulence factors under appropriate cell density conditions. Synthesized as a propeptide, this fusion molecule is exported from the cell via the secA pathway, cleaved by an extracellular protease and re-imported into the cell via an oligopeptide permease. Both the extracellular protease as well as olidopeptide permease are ubiquitous structures to the Gram-positive cell wall. A such, any gram-positive bacteria expressing the PlcR-PapR protein should be able to both process as well as import the activating form of the fusion peptide.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Improvement:TMMU-China 2017

Author：Yizhen Xu

Summary：In this improvement, we splited this part into two parts that can be used in cell-cell communication.

From reference [1], the PlcR-PapR fusion peptide can activate gene expression without the PapR signal peptide. Because alteration of the export signal, this fusion peptide can not be exported and re-imported into the cell to activate the PlcR transcription factor. In our improvement, we provided two new parts. One is the PlcR protein (< a href="https://parts.igem.org/Part:BBa_K2279002">BBa_K2279002</ a>), the other is PapR signal peptide (< a href="https://parts.igem.org/Part:BBa_K2279003">BBa_K2279003</ a>). Combining these two new parts, we can build autoinduction system and Sender-Receiver Cells to enable cell-cell communication.

Reference

[1] Pomerantsev A.P., Pomerantseva O.M. & Leppla S.H. (2004), A Spontaneous Translational Fusion of Bacillus cereus PlcR and PapR Activates Transcription of PlcR-Dependant Genes in Bacillus anthracis via Binding with a Specific Palindromic Sequence. Infection and Immunity 72:5814-5823.