Composite

Part:BBa_K2423000:Design

Designed by: iGEM Uppsala 2017   Group: iGEM17_Uppsala   (2017-08-18)
Revision as of 12:24, 27 October 2017 by Greijer (Talk | contribs) (→‎References)


CaCCD2 under the control of a constitutive promoter (BBa_J23106)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1774
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 672


Design Notes

We made a complete device including promoter and RBS. We also included a 6x his tag for easy purification of the expressed protein.


Source

Crocus ancyrensis

References

1. Frusciante S, Diretto G, Bruno M, Ferrante P, Pietrella M, Prado-Cabrero A, et al. Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis. Proc Natl Acad Sci USA. 2014 Aug 19;111(33):12246–51.

2. Ahrazem O, Rubio-Moraga A, Berman J, Capell T, Christou P, Zhu C, et al. The carotenoid cleavage dioxygenase CCD2 catalysing the synthesis of crocetin in spring crocuses and saffron is a plastidial enzyme. New Phytol. 2016 Jan 1;209(2):650–63.

3. Crocus ancyrensis carotenoid cleavage dioxygenase (CCD2) mRNA, complete cds. 2015 Nov 4; Available from: http://www.ncbi.nlm.nih.gov/nuccore/KP792756.1