Composite

Part:BBa_K2423001

Designed by: iGEM Uppsala 2017   Group: iGEM17_Uppsala   (2017-08-18)
Revision as of 12:09, 27 October 2017 by Greijer (Talk | contribs)


CsADH-2946 under the control of a constitutive promoter (BBa_J23106)

This part codes for aldehyde dehydrogenase 2946, which catalyses the conversion of crocetin dialdehyde into crocetin, using NAD+ as a cofactor, in Crocus sativus. It contains a constitutive promoter, as well as a His tag for easy prification. A version of this biobrick containing an inducible promoter is BBa_K2423007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 602
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1119
    Illegal SapI.rc site found at 227


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Categories
Parameters
None