Composite

Part:BBa_K2423000

Designed by: iGEM Uppsala 2017   Group: iGEM17_Uppsala   (2017-08-18)
Revision as of 12:05, 27 October 2017 by Greijer (Talk | contribs)


CaCCD2 under the control of a constitutive promoter (BBa_J23106)

This part codes for carotenoid cleavage dioxygenase 2, a member of the CCD family. This enzyme catalyses the conversion of zeaxanthin into crocetin dialdehyde in Crocus ancyrensis through cleavage at the 7,8 and 7',8' position. The part contains a constitutive promoter, and a His tag for purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1774
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 672


[edit]
Categories
Parameters
None