Help:Cell-free chassis/Modelling

< Help:Cell-free chassis
Revision as of 15:05, 23 October 2007 by Vincent (Talk | contribs)

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Cell-Free System modelling

Model assumptions

  • Simple constitutive gene expression model
  • Protein degradation is negligeable.
  • Resources are an important limiting factor to be considered in our CFS ( Energy or DNTPs related)
  • + ( ODE related assumptions)

Model description

  • mathematical description of the system + parameter info.
  • see [http://openwetware.org/wiki/User:Jaroslaw_Karcz/Sandbox]
  • link to SBML file

Simulations: typical beahviours

  • weak promoter with no degradation (linear production of the output protein) ---> like the pTet data we have
  • strong promoter with no degradation (resources are limiting the rate of synthesis as well as the amount of protein to be produced) ---> like the pT7 data we have