Part:BBa_C0062

luxR repressor/activator, (no LVA?)

In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr BBa_R0062, and repressing transcription from Pl BBa_R0063.

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.

Usage and Biology

We improved this part by using it in a part which allows for the constitutive expression of LuxR (BBa_K1893004). This part also includes the pLux promoter, so that genes placed downstream the promoter will be expressed when induced with 3OC6-AHL. We also made a part which allows for the characterisation of LuxR, as we placed GFP downstream the pLux promoter (BBa_K1893005). The part is currently being characterised.

Unspecified.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Contribution: SiCAU-China 2017

Authors:Wenwen Lin

Summary:The QS system LuxR/LuxI has been widely used in kinds of logic circuit. But this year we find that LuxR can not always work well in E.coli. The period that used to induce is logarithmic phase, however, the LuxR, in E.coli, will not work when E.coli enter into stationary phase. Users who will use LuxR as an activating transcription may be aware of its feasibility by this message.