Composite

Part:BBa_K2217023:Design

Designed by: Mohammad Tarek Mansour   Group: iGEM17_AFCM-Egypt   (2017-09-24)
Revision as of 16:50, 23 October 2017 by MTarek (Talk | contribs)


YFP for ceRNA circuit characterization


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part BBa_K2217023 is composed of lac operator (BBa_K2217016) and the lac promoter (BBa_K2217017) as a weak constitutive promoter in a trial to improve characterization of ceRNA network using yfb (BBa_K2217014) detection using flow cytometry. Finally, it was terminated by Poly_gh termination (BBa_K2217015).



Source

IDT synthesized fragment

References