Reporter

Part:BBa_K2314024

Designed by: Xizhong Ding   Group: iGEM17_OUC-China   (2017-10-22)
Revision as of 03:44, 23 October 2017 by Ykkchen (Talk | contribs)


yEcitrine is one of the improved variants of YFP. It has been widely used in yeast.

yEcitrine is one of the improved variants of YFP. It has been widely used in yeast and has a good performance than unoptimized YFP. The excitation/emission pair (in nm) is 502/532. Ref: Sheff, Mark A., and Kurt S. Thorn. "Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae." Yeast 21.8 (2004): 661-670.

The structure of YEc


T--OUC-China--INPmSA-yEC.gif


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
//chassis/eukaryote/yeast
//function/reporter
//function/reporter/fluorescence
Parameters
emission502nm
excitation532nm