Coding

Part:BBa_K2332316

Designed by: Camillo Moschner   Group: iGEM17_UCL   (2017-10-22)
Revision as of 23:52, 22 October 2017 by CamilloMoschner (Talk | contribs)


dCas9-p65 (TF)

Plasma Membrane Delivery System (PMDS)
Function Delivery of protein (e.g. mCherry) to the PM
Use in Mammalian cells
Abstraction Hierarchy Part
RFC standard RFC10, RFC12, RFC23 & RFC25 compatible
Backbone pSB1C3
Submitted by [http://2017.igem.org/Team:UCL UCL iGEM 2017]

This gene encodes nuclease deactivated Cas9 (dCas9) fused to the transactivation domain of NFκB, called ReLa p65. This protein allows targeted expression of endogenous and exogenous expression of genes via association of co-translated gRNA's.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5011
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

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[edit]
Categories
Parameters
None