Composite

Part:BBa_K2203001:Design

Designed by: Lena Bruhin   Group: iGEM17_EPFL   (2017-10-22)
Revision as of 18:37, 22 October 2017 by Lenabruhin (Talk | contribs) (→‎Design Notes)

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T7-GamS-HisTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 336
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 323
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

extension PCR to add T7 promoter and terminator and the 6XHis-tag to the gamS coding sequence(BBa_K2203000)

Source

pBADmod1-linker2-gamS

References

https://www.addgene.org/45833/