Cell-free chassis/Commercial E. coli T7 S30

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Commercial E. coli T7 S30

Cell-Free Systems Chassis description Optimum temperature Peak time Product stability Expression capacity Expression lifespan

Description

The Commercial E. coli T7 S30 extract was purchased from Promega. It was prepared by modifications of the method described by Zubay et al (1980). E. coli strain B deficient in OmpT endoproteinase and lon protease activity was used.

The simple constitutive gene expression device BBa_E7104 has been used to characterize this cell-free chassis

References

Please refer to the [http://www.promega.com/tbs/tb219/tb219.pdf Promega cell extract user guide] for detailed information about the Commercial E. coli T7 S30 extract.