Composite

Part:BBa_K2350021:Design

Designed by: YA-XUAN YANG   Group: iGEM17_NYMU-Taipei   (2017-10-22)
Revision as of 08:03, 22 October 2017 by Yang20051 (Talk | contribs)


R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012-J23118-B0034-NrtA-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1035
    Illegal NheI site found at 1058
    Illegal NheI site found at 1757
    Illegal NheI site found at 1780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2990
    Illegal BamHI site found at 2360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1362
    Illegal AgeI site found at 1432
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

　　In our 2017 NYMU project, the endolysin and holin both are from enterobacteria phage T4, for the reason that we don’t know whether endolysin and holin from different species could cooperate well.

Source

　　The endolysin and holing we used are from iGEM released parts, BBa_K112806 and BBa_K112000, respectively. NrtA is from cyanobacteria Synechocystis sp. PCC 6803.

References

  • 2008 UC Berkeley Team: http://2008.igem.org/Team:UC_Berkeley
  • 2014 Peking Team: http://2014.igem.org/Team:Peking/Suicide