DNA

Part:BBa_K2267000

Designed by: HE, BOTAO   Group: iGEM17_TUST_China   (2017-10-21)
Revision as of 22:53, 21 October 2017 by Registry (Talk | contribs)

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a pH sensitive promoter Pasr

GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH.https://static.igem.org/mediawiki/parts/8/8d/GlsA%E6%8F%92%E5%9B%BE.docx

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 235
    Illegal SpeI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 235
    Illegal SpeI site found at 2
    Illegal NotI site found at 9
    Illegal NotI site found at 227
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 235
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 235
    Illegal SpeI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 235
    Illegal SpeI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None