Cell-free chassis/Commercial E. coli T7 S30
Commercial E. coli T7 S30
Optimum temperature | Peak time | Product stability | Expression capacity | Expression lifespan |
Description
The Commercial E. coli T7 S30 extract was purchased from Promega. It was prepared by modifications of the method described by Zubay et al (1980). E. coli strain B deficient in OmpT endoproteinase and lon protease activity was used.
The simple constitutive gene expression device BBa_E7104 has been used to characterize this cell-free chassis
References
Please refer to the Promega cell extract user guide for detailed information about the Commercial E. coli T7 S30 extract.