Composite

Part:BBa_K2250005

Designed by: Shuailin Li   Group: iGEM17_Tsinghua-A   (2017-10-19)
Revision as of 07:56, 19 October 2017 by Shuailin10 (Talk | contribs)


Pconst-RhlR-Prhl-LacI-PlacI-CmR

This is correspond to the reception part of warrior 2/beggar 2/farmer 2 in our project. This part make warrior 2/beggar 2/farmer 2 able to recept the C4HSL secreted by warrior 1 so that the warrior 1 can kill warrior 1/beggar 1/farmer 1. This can be realized by C4HSL binding to RhlR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 2/beggar 2/farmer 2 unable to survive under the Chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2159
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776


[edit]
Categories
Parameters
None