Coding

Part:BBa_K2443050

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-10)
Revision as of 14:57, 18 October 2017 by Graeme.glaister (Talk | contribs)

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Ile RS

Isoleucine tRNA synthetase responsible for attaching isoleucine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal histidine tag with a serine glycine linker.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1370
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 591
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 98
    Illegal SapI.rc site found at 1489


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Categories
Parameters
None