Composite

Part:BBa_K2404016

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-10-16)
Revision as of 11:11, 16 October 2017 by Registry (Talk | contribs)

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A TSH antagonist with His-Tags, controlled by the LexA promoter

A thyroid stimulating hormone antagonist, with His-Tags for purification, under the control of the LexA XVE-inducible promoter native to E. coli . This has the Nopaline synthase gene terminator from Agrobacterium tumefaciens . The construct is contained within the pGB-A2 plasmid. It should be used to produce the TSH antagonist under control of XVE.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1143
    Illegal XhoI site found at 955
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None