Part:BBa_K2443044

Cys RS

Cysteine tRNA synthetase responsible for attaching cysteine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal histidine tag with a serine glycine linker.

Amino acid composition: Ala (A) 49 10.3% Arg (R) 30 6.3% Asn (N) 18 3.8% Asp (D) 38 8.0% Cys (C) 5 1.1% Gln (Q) 16 3.4% Glu (E) 34 7.2% Gly (G) 37 7.8% His (H) 22 4.6% Ile (I) 21 4.4% Leu (L) 36 7.6% Lys (K) 23 4.8% Met (M) 21 4.4% Phe (F) 19 4.0% Pro (P) 15 3.2% Ser (S) 21 4.4% Thr (T) 18 3.8% Trp (W) 7 1.5% Tyr (Y) 16 3.4% Val (V) 29 6.1% Pyl (O) 0 0.0% Sec (U) 0 0.0%

(B)   0	  0.0%
(Z)   0	  0.0%
(X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 72 Total number of positively charged residues (Arg + Lys): 53

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 993
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 859