Composite

Part:BBa_K2423005:Design

Designed by: Oscar Broström   Group: iGEM17_Uppsala   (2017-10-01)
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CaCCD2 with BBa_J04500


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 837


Design Notes

When designing this part we initially took the coding sequence from KP792756 and codon optimized it for Escherichia Coli using IDT's tool. We repeatedly used it until there were no restriction sites for EcoRI, XbaI, SpeI and PstI. The choice of J04500 came from that we wanted to be able to induce the production of our protein using IPTG.


Source

Originally, the group enzymes was discovered by Frusciante et al. (2014) through deep transcriptome analysis. More specific information about CaCCD2 came approximately one year later Ahrazem et al. (2015). The sequencing they found there has the GenBank acession number KP792756.

References