Coding
SpoIID

Part:BBa_K2273066:Design

Designed by: Henri Deda   Group: iGEM17_TU_Dresden   (2017-09-22)
Revision as of 18:51, 22 September 2017 by Henrideda (Talk | contribs) (→‎Source)


SpoIID signal peptide of B. subtilis lytic transglycosylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

tba


The SpoIID signal peptide of B. subtilis lytic transglycosylase was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

SpoIID_SP fwd gatcGAATTCGCGGCCGCTTCTAGATAAGGAGGTCAAAAATGAAACAATTCGCAATCACACTAT
SpoIID_SP rev gatcTCTGCAGCGGCCGCTACTAGTATTAACCGGTGGCCCCCGCTTCCTTATT

Following amplification, the signal peptide was digested using EcoRI and PstI and ligated into pSB1C3.

References