Regulatory
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Part:BBa_K2404002

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-09-22)
Revision as of 17:33, 22 September 2017 by Ryancoates (Talk | contribs)


A Thyroid Stimulating Hormone antagonist without His-Tags

This is the most 3’ 500bp of an inducible promoter that has been isolated from Arabidopsis thaliana . It responds to jasmonic acid. This part is suitable for Golden Gate cloning using type IIS restriction enzymes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 794


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Categories
Parameters
None