Coding
TSHantag

Part:BBa_K2404000:Design

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-09-12)
Revision as of 13:52, 12 September 2017 by Registry (Talk | contribs)

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A Thyroid Stimulating Hormone antagonist without His-Tags


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 794


Design Notes

To produce this protein we needed to create a gene construct with the TSH antagonist as the coding sequence. We used the Golden Gate cloning system to do this, and so needed to create upstream restriction enzyme recognition sites (i.e. Type IIS restriction endonucleases) that are suitable for Golden Gate reactions.


Source

Synthetic G-block. This modified protein was discovered in a paper by Fares et al in 2001.

References